Modified transposases for improved insertion sequence bias and increased DNA input tolerance

US10385323B2 · kind B2 · utility

Assignee

• Illumina, Inc. · US

Inventors

• Christian Gloeckner · Bonn, DE
• Amirali Kia · San Mateo, US
• Erin Bomati · Santee, US
• Molly He · San Diego, US
• Haiying Li Grunenwald · Belleville, US
• Scott Kuersten · Madison, US
• Trina Faye Osothprarop · San Diego, US
• Darin Haskins · Madison, US
• Joshua Burgess · Sun Prairie, US
• Anupama Khanna · Madison, US
• Daniel Schlingman · Madison, US
• Ramesh Vaidyanathan · Madison, US

Key dates

• Filing date: Jun 28, 2018
• Grant date: Aug 20, 2019
• Expiry date: Jun 28, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6869
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Presented herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction conditions which exhibit improved insertion sequence bias, as well as methods and kits using the same.