Patent · US Active

Programmable RNA shredding by the type III-A CRISPR-Cas system of Streptococcus thermophilus

US10385336B2 · kind B2 · utility

1Cited by

0References

14Claims

0Family size

Assignee

Vilnius University · LT

Inventors

Virginijus Siksnys · Vilnius, LT
Migle Kazlauskiene · Vilnius, LT
Gintautas Tamulaitis · Vilnius, LT

Key dates

Filing date	Mar 3, 2017
Grant date	Aug 20, 2019
Priority date	—
Expiry date	Mar 27, 2037

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2310/20
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A Type III-A CRISPR-Cas (StCsm) complex of Streptococcus thermophilus comprising crRNA, Csm4, and Csm3 and use for cleavage of RNA bearing a nucleotide sequence complementary to the crRNA, in vitro or in vivo. Methods for site-specific cleavage/shredding of a target RNA molecule using an RNA-guided RNA endonuclease comprising a minimal complex of crRNA, Csm4, and Csm3, and methods of RNA knock-down and RNA knock-out are disclosed.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.