Microfluidic electrocage device and cell medium for trapping and rotating cells for live-cell computed tomography (CT)

US10391485B2 · kind B2 · utility

Assignee

• ARIZONA BOARD OF REGENTS, A BODY CORPORATE OF THE STATE OF ARIZONA ACTING FOR AND ON BEHALF OF ARIZONA STATE UNIVERSITY · US

Inventors

• Deirdre Meldrum · Phoenix, US
• Roger Johnson · Phoenix, US
• Iniyan Soundappa Elango · Lehi, US
• Andrew Shabilla · Scottsdale, US
• Hong Wang · Cypress, US
• Jakrey Myers · Scottsdale, US
• Laimonas Kelbauskas · Gilbert, US
• Dean Smith · Spanaway, US
• Pimwadee Limsirichai · Chandler, US

Key dates

• Filing date: Sep 25, 2014
• Grant date: Aug 27, 2019
• Expiry date: Nov 20, 2035

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01L2300/0645
• WIPO field: Surface technology, coating
• WIPO sector: Chemistry

Abstract

A microfluidic device useable for performing live cell computed tomography imaging is fabricated with a cover portion including a first wafer with at least one metal patterned thereon, a base portion including a second wafer with at least one metal patterned thereon and negative photoresist defining recesses therein, and a diffusive bonding layer including a negative photoresist arranged to join the cover portion and the base portion. A composition useful in live cell computer topography includes a long-chain polysaccharide at a concentration of from about 0.01% to about 10.0% in cell culture medium for supporting cell life while enabling cell rotation rate to be slowed to a speed commensurate with low light level imaging.