System and method for isolating and analyzing cells

US10391492B2 · kind B2 · utility

Assignee

• Celsee Diagnostics, Inc. · US

Inventors

• Kalyan Handique · Ann Arbor, US
• Vishal Sharma · Kanchinakote, IN
• Priyadarshini Gogoi · Plymouth, US
• William Weiyeh Chow · Los Angeles, US
• Austin Payne · Ann Arbor, US
• Kyle Gleason · Hercules, US
• Brian Boniface · Plymouth, US
• John Connolly · Lynbrook, US
• Sam Tuck · Hercules, US

Key dates

• Filing date: Aug 28, 2018
• Grant date: Aug 27, 2019
• Expiry date: Aug 28, 2038

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2015/1006
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: distributing a population of cells and a population of non-cell particles across the array of wells through the fluid reservoir to increase capture efficiency of individual cell-particle pairs within the array of wells, and processing the captured cell-particle pairs at the set of wells.