Patent · US Active

Methods of single-cell barcoding and sequencing

US10392614B2 · kind B2 · utility

7Cited by
110References
19Claims
0Family size

Assignee

Inventors

Key dates

Filing dateSep 29, 2017
Grant dateAug 27, 2019
Priority date—
Expiry dateSep 29, 2037

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q2600/16
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

Provided herein are methods for immune repertoire sequencing and single cell barcoding. In some aspects, such methods may comprise producing a plurality of cDNAs from a plurality of polynucleotides, wherein at least one polynucleotide encodes a heavy and/or light chain, from a plurality of immune cells from a biological sample. Producing a plurality of cDNAs may comprise reverse transcription and template switching, such as to form a plurality of uniquely barcoded cDNAs. Methods described herein further comprise amplifying the plurality of uniquely barcoded cDNAs, thereby forming a library, and sequencing one or more of the sequences of the library.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.