Method and kit for determining the genome integrity and/or the quality of a library of DNA sequences obtained by deterministic restriction site whole genome amplification

US10392657B2 · kind B2 · utility

Assignees

• Menarini Silicon Biosystems S.p.A. · IT
• Fraunhofer-Gesellschaft zur förderung der angewandten Forschung e.V. · DE

Inventors

• Christoph Klein · Darmstadt, DE
• Bernhard Michael Polzer · Munich, DE
• Nicolò Manaresi · Bologna, IT

Key dates

• Filing date: Dec 4, 2014
• Grant date: Aug 27, 2019
• Expiry date: Nov 12, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2545/101
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

A method for determining the integrity of the genome of a sample and/or the quality of a library of DNA sequences obtained by deterministic restriction site whole genome amplification can include (a) amplifying the library of DNA sequences to produce first, second, and third PCR products each of a different size from 50 bp to 1000 bp, by PCR using at least one first primer pair, one second primer pair and one third primer pair, the primer pairs each hybridizing to a DNA sequence of the library having a length from 1000 bp to 5000 bp and corresponding to a sequence of the genome located respectively on a first, second and third chromosome arm; (b) detecting the first, second and third PCR products; (c) correlating the presence of the first, second and third PCR products with the integrity of the genome of the sample and/or the quality of the library.