Attenuated virus having multiple hosts

US10400220B2 · kind B2 · utility

Assignee

• The Research Foundation for The State University of New York · US

Inventors

• Eckard Wimmer · Port Jefferson, US
• Steffen Mueller · Pforzheim, DE
• Bruce Futcher · Stony Brook, US
• Sam Shen · Iowa City, US
• Charles Stauft · Setauket, US
• Charles A. Ward · Toronto, CA

Key dates

• Filing date: Sep 8, 2015
• Grant date: Sep 3, 2019
• Expiry date: Sep 8, 2035

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A50/30
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

This invention provides an attenuated virus comprising a modified viral genome engineered to containing multiple nucleotide substitutions that reduce the codon pair bias of a virus protein encoding sequence relative to a first host while the codon pair bias relative to a second host is not substantially reduced. In another embodiment, the invention provides an attenuated virus comprising modified viral genome engineered to containing multiple nucleotide substitutions that reduce the codon pair bias of a virus protein-encoding sequence relative to a first host and a second host. The attenuated virus may be used in a vaccine composition for inducing a protective immune response in a subject. The invention also provides a method of synthesizing the attenuated virus. Further, this invention further provides a method for preventing a subject from becoming afflicted with a virus-associated disease comprising administering to the subject a prophylactically effective dose of a vaccine composition comprising the attenuated virus.