Truncated pullulanases, methods of production, and methods of use thereof
US10400228B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 10, 2015 |
| Grant date | Sep 3, 2019 |
| Priority date | — |
| Expiry date | Nov 20, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/01041
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Truncated pullulanases having an N-terminal deletion in a parental pullulanase are provided. These truncated pullulanases have altered properties as compared to the parental pullulanase, including an increased saccharification rate and higher catalytic activity at acidic pH values below 4.5 and higher temperatures of up to 64° C. These truncated pullulanase also have improved thermal stability as compared to the parental enzyme. Also provided are compositions containing the truncated pullulanases and a glucoamylase, and processes for applying the truncated pullulanases and compositions in the starch industry. Polynucleotides that encode the truncated pullulanases, and recombinant host cells for producing the truncated pullulanases are also described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.