Nanopore-based single DNA molecule characterization, identification and isolation using speed bumps
US10400278B2 · kind B2 · utility
1Cited by
113References
18Claims
0Family size
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Key dates
| Filing date | Feb 16, 2017 |
| Grant date | Sep 3, 2019 |
| Priority date | — |
| Expiry date | Jun 19, 2037 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S977/924
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to a method of using nanopores to obtain sequence information of sample DNAs in ss test DNAs. The method comprises using speed bumps to stall the ss test DNAs in the nanopores at random positions of the ss test DNAs to obtain sequence information of each and every nucleotides of the sample DNAs, and to construct the whole sequences of the sample DNAs. The present invention also relates to identification and/or isolation of test DNAs having desired sequence(s) using nanopore detectors facilitated by speed bump.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.