Use of multi-arm polyethylene glycol modifier and application of multi-arm polyethylene glycol modifier in L-asparaginasum modification

US10406235B2 · kind B2 · utility

Assignees

• ZONHON BIOPHARMA INSTITUTE, INC. · CN
• GENSUN INSTITUTE OF BIOMEDICINE CO., LTD. · CN

Inventors

• Bruce Yong MA · 安丰镇, CN
• Jun Wang · Nanjing, CN
• Dinglong Wu · 安丰镇, CN
• Chunlin Xu · 安丰镇, CN
• Yaofang Wang · 安丰镇, CN

Key dates

• Filing date: Jul 28, 2014
• Grant date: Sep 10, 2019
• Expiry date: Nov 29, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y305/01005
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods for use of a multi-arm polyethylene glycol (PEG) modifier in modification of asparaginase. The described multi-arm PEG modifier enhances the subunit interaction of a multimeric protein to maintain the multimeric protein in a polymerized form, thereby improving the stability of the multimeric protein, maintaining the bioactivity of the multimeric protein, and reducing the probability of exposure of the antigen binding site after depolymerization of the subunits, so as to reduce the immunogenicity.