Methods for glycoprotein remodeling using endoglycosidase mutants
US10407673B2 · kind B2 · utility
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Key dates
| Filing date | Jun 18, 2018 |
| Grant date | Sep 10, 2019 |
| Priority date | — |
| Expiry date | Jun 18, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/21
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A mutant of EndoS2 includes one or more mutations in the sequence of a wild-type EndoS2 (SEQ ID NO:1), wherein the one or more mutations are in a peptide region located within residues 133-143, residues 177-182, residues 184-189, residues 221-231, and/or residues 227-237, wherein the mutant of EndoS2 has a low hydrolyzing activity and a high tranglycosylation activity, as compared to those of the wild-type EndoS2. A method for preparing an engineered glycoprotein using the mutant of EndoS2 includes coupling an activated oligosaccharide to a glycoprotein acceptor. The activated oligosaccharide is a glycan oxazoline.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.