Method for direct transformation of exogenous DNA into resting spores of Aspergillus niger independent of mediators
US10407684B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Oct 21, 2016 |
| Grant date | Sep 10, 2019 |
| Priority date | — |
| Expiry date | Oct 21, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/66
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention discloses a method for direct transformation of exogenous DNA into resting spores of Aspergillus niger independent of mediators. The method includes three steps of culture of Aspergillus niger and collection of spores, pretreatment of Aspergillus niger spores, and electroporation of Aspergillus niger spores by using HDEN method, to obtain Aspergillus niger spores with introduction of plasmids to be transformed. In the present invention, non-germinated spores are used as a starting material for introduction of an exogenous molecule, and exogenous DNA is introduced into the resting spores of Aspergillus niger by employing the HDEN electrotransformation technique, whereby the complex step of spore germination is omitted, and steps of protoplast preparation or Agrobacterium-mediated transformation in conventional methods etc. are omitted. Moreover, the transformation efficiency is high, and at least an effect of no less than 6000 positive transformants per transformation reaction system can be achieved.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.