Allele-specific capture of nucleic acids

US10415029B2 · kind B2 · utility

Assignee

• OMNIOME, INC. · US

Inventors

• Corey M. Dambacher · Village of La Jolla, US
• Eugene Tu · San Diego, US

Key dates

• Filing date: Sep 11, 2017
• Grant date: Sep 17, 2019
• Expiry date: May 30, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6869
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for separating a target allele from a mixture of nucleic acids by (a) providing a mixture of nucleic acids in fluidic contact with a stabilized ternary complex that is attached to a solid support, wherein the stabilized ternary complex includes a polymerase, primed nucleic acid template, and next correct nucleotide, wherein the template has a target allele, wherein the next correct nucleotide is a cognate nucleotide for the target allele, and wherein the stabilized ternary complex is attached to the solid support via a linkage between the polymerase and the solid support or via a linkage between the next correct nucleotide and the solid support; and (b) separating the solid support from the mixture of nucleic acids, thereby separating the target allele from the mixture of nucleic acids.