Pseudotyping of retroviral vectors, methods for production and use thereof for targeted gene transfer and high throughput screening

US10415057B2 · kind B2 · utility

Assignee

• BUNDERSREPUBLIK DEUTSCHLAND, LETZVERTRETEN DURCH DEN PRÄSIDENTEN DES PAUL-EHRLICH-INSTITUTS · DE

Inventors

• Christian Buchholz · Gütersloh, DE
• Sabrina Kneissl · Darmstadt, DE
• Klaus Cichutek · Langen, DE
• Roberto Cattaneo · Rochester, US

Key dates

• Filing date: Dec 22, 2017
• Grant date: Sep 17, 2019
• Expiry date: Dec 22, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2810/859
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention relates to the pseudotyping of retroviral vectors with heterologous envelope proteins derived from the Paramyxoviridae family, genus Morbillivirus, and various uses of the resulting vector particles. The present invention is based on the unexpected and surprising finding that the incorporation of morbillivirus F and H proteins having truncated cytoplasmic tails into lentiviral vector particles, and the complex interaction of these two proteins during cellular fusion, allows for a superior and more effective transduction of cells. Moreover, these pseudotyped vector particles allow the targeted gene transfer into a given cell type of interest by modifying a mutated and truncated H protein with a single-chain antibody or ligand directed against a cell surface marker of the target cell.