Detection method for low molecular weight heparin complete degradation products using hydrophilic interaction chromatography and multiple reaction monitoring tandem mass spectrometry
US10416132B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 27, 2017 |
| Grant date | Sep 17, 2019 |
| Priority date | — |
| Expiry date | Jun 9, 2037 |
Classification
- Technology area (CPC H)Electricity
- CPC primaryH01J49/4215
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A detection method for low molecular weight heparin complete degradation products using hydrophilic interaction chromatography and multiple reaction monitoring tandem mass spectrometry. Identifying the original reducing end and non-reducing end of enoxaparin sodium by means of reducing the reducing end of enoxaparin sodium, and performing hydrolysis using hydrogen peroxide. Performing quantitative analysis on all component units utilizing hydrophilic interaction chromatography and multiple reaction monitoring tandem mass spectrometry, in particular quantifying low-content special structures and characterizing low molecular weight heparin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.