Method of deep tissue imaging using multi-photon excitation of a fluorophore

US10420471B2 · kind B2 · utility

Assignee

• RESEARCH FOUNDATION OF THE CITY UNIVERSITY OF NEW YORK · US

Inventors

• Robert R. Alfano · New York, US
• Yang Pu · Beihai, CN
• Lingyan Shi · New York, US
• Sebastião Pratavieira · São Paulo, BR

Key dates

• Filing date: Jun 9, 2014
• Grant date: Sep 24, 2019
• Expiry date: Apr 4, 2038

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N21/63
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method is provided for deep tissue imaging using multi-photon excitation of a fluorescent agent. The fluorescent agent is irradiated with an ultrafast laser to produce an excited singlet state (Sn) which subsequently undergoes non-radiative relaxation to a first singlet state (S1). The S1 state undergoes fluorescence to the ground state S0 to produce an emission wavelength. Both the excitation and emission wavelengths are within the near infrared optical window, thereby permitting deep tissue penetration for both the incoming and outgoing signals.