Methods of screening, selecting, and identifying cytotoxic recombinant polypeptides based on an interim diminution of ribotoxicity
US10421958B2 · kind B2 · utility
10Cited by
6References
23Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 4, 2015 |
| Grant date | Sep 24, 2019 |
| Priority date | — |
| Expiry date | Feb 4, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/20
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods of screening libraries of chimeric molecules comprising ribotoxic polypeptides, where screening is based on the interim reduction or elimination of ribotoxicity and the methods can identify cytotoxic molecules, each comprising a binding region and a ribotoxic region which jointly possess a desired assay-selectable characteristic, such as, e.g., binding to a target biomolecule, binding to a target cell, and/or cellular internalization.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.