Rapid epidemiologic typing of bacteria
US10421991B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Nov 11, 2015 |
| Grant date | Sep 24, 2019 |
| Priority date | — |
| Expiry date | Jan 22, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/16
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for typing a strain of an organism are provided, the methods comprising the steps of amplifying, in a single reaction mixture containing nucleic acid from the organism, dividing the reaction mixture into a plurality of sets of second-stage reaction wells, each set of second-stage reaction wells containing a different pair of second-stage primers, subjecting each of the second-stage reaction wells to amplification conditions to generate a plurality of second-stage amplicons, melting the second-stage amplicons to generate a melting curve for each second-stage amplicon, and identifying the strain of the organism from the melting curves.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.