Methods and compositions for DNA profiling

US10422002B2 · kind B2 · utility

Assignee

• Illumina, Inc. · US

Inventors

• Kathryn M. Stephens · Poway, US
• Cydne Holt · San Diego, US
• Carey Davis · San Diego, US
• Anne Jager · San Diego, US
• Paulina Walichiewicz · San Diego, US
• Yonmee Han · San Diego, US
• David Silva · Nipomo, US
• Min-Jui Richard Shen · Boyds, US
• Sasan Amini · Redwood City, US
• Frank J. Steemers · Encinitas, US

Key dates

• Filing date: Feb 13, 2015
• Grant date: Sep 24, 2019
• Expiry date: Sep 16, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/16
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Embodiments disclosed herein provide methods for constructing a DNA profile comprising: providing a nucleic acid sample, amplifying the nucleic acid sample with a plurality of primers that specifically hybridize to at least one target sequence comprising a SNP and at least one target sequence comprising a tandem repeat, and determining the genotypes of the at least one SNP and at least one tandem repeat in the amplification products, thereby constructing the DNA profile of the nucleic acid sample. Embodiments disclosed herein further provide a plurality of primers that specifically hybridize to at least one short target sequence and at least one long target sequence in a nucleic acid sample, wherein amplifying the nucleic acid sample using the plurality of primers in a single reaction results in a short amplification product and a long amplification product, wherein each of the plurality of primers comprises one or more tag sequences.