Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes

US10428362B2 · kind B2 · utility

Assignee

• E.I. DU PONT DE NEMOURS AND COMPANY · US

Inventors

• Kevin D. Nagy · Wilmington, US
• Erwin F. Columbus-Hago · Sunnyvale, US
• Jayarama K. Shetty · Pleasanton, US
• Susan Marie Hennessey · Avondale, US
• Robert DiCosimo · Chadds Ford, US
• Ling Hua · Shanghai, CN
• Rodrigo Ramirez · Campinas, BR
• Zhongmei Tang · Shanghai, CN
• Zheyong Yu · Shanghai, CN

Key dates

• Filing date: May 10, 2018
• Grant date: Oct 1, 2019
• Expiry date: May 10, 2038

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02E50/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method is disclosed for hydrolyzing an alpha-1,5 glucosyl-fructose linkage in a saccharide (disaccharide or oligosaccharide) such as leucrose. This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase or glucoamylase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,5 glucosyl-fructose linkage of the saccharide. This method is useful for reducing the amount of leucrose in a filtrate isolated from a glucan synthesis reaction, for example.