Sequencing method for rapid identification and processing of cognate nucleotide pairs
US10428378B2 · kind B2 · utility
5Cited by
38References
27Claims
0Family size
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Key dates
| Filing date | Jul 19, 2017 |
| Grant date | Oct 1, 2019 |
| Priority date | — |
| Expiry date | Feb 8, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2001/4038
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided are methods and systems for reducing the time needed for sequencing nucleic acids. The approach relies on detecting formation of nucleotide-specific ternary complexes comprising a polymerase (e.g., a DNA polymerizing enzyme), a primed template nucleic acid molecule, and a nucleotide complementary to the templated base of the primed template nucleic acid. The methods and systems facilitate determination of the next correct nucleotide, as well as the subsequent next correct nucleotide from a cycle of examining four different nucleotides without requiring chemical incorporation of any nucleotide into the primer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.