Methods and compositions for targeted polynucleotide modification

US10443064B2 · kind B2 · utility

Assignee

• PIONEER HI-BRED INTERNATIONAL, INC. · US

Inventors

• William J. Gordon-Kamm · Urbandale, US
• Keith Lowe · Johnston, US
• David J. Peterson · Johnston, US
• Christopher J. Scelonge · Ankeny, US
• Grace M. St. Clair · Des Moines, US
• Bing-Bing Wang · Johnston, US

Key dates

• Filing date: Feb 7, 2018
• Grant date: Oct 15, 2019
• Expiry date: Mar 6, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/415
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A variety of methods and compositions are provided, including methods and compositions for targeted modification of a specific target site in a cell or organism, methods for integrating polynucleotides of interest, methods to assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, silence a gene, and identify and/or characterize transcriptional regulating regions. The methods involve the introduction of a cell proliferation factor and a double-strand break-inducing enzyme into an organism.