Methods and materials for obtaining hepatocyte lineage cells
US10443071B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jan 27, 2017 |
| Grant date | Oct 15, 2019 |
| Priority date | — |
| Expiry date | Jun 22, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2800/40
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods of producing hepatocyte lineage cells are provided. The method can include transfecting a cell with one or more expression vectors. For example, a cell can be transfected with a first expression vector containing a first gene that encodes CCAAT/enhancer binding protein alpha (CEBPA), a second expression vector containing a second gene that encodes CCAAT/enhancer binding protein beta (CEBPB), a third expression vector containing a third gene that encodes forkhead box A1 (FOXA1), and a fourth expression vector containing a fourth gene that encodes forkhead box A3 (FOXA3). The method can include culturing the transfected cell obtained in a growth environment. The transfected cell can be cultured in Williams' E medium, ReproFF (feeder-free media maintaining pluripotency) medium, or both. Transfected and/or hepatocyte lineage cells obtained by a method of the present invention are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.