Method for determining the concentration of glucoraphanin and/or sulforaphane in a plant
US10444212B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Feb 24, 2017 |
| Grant date | Oct 15, 2019 |
| Priority date | — |
| Expiry date | Apr 13, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/12
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
In a method for determining the concentration of glucoraphanin in a plant a sample of the plant is contacted by a polar uncharged organic solvent and comminuted such that glucoraphanin and plant material contained in the sample are dissolved in the solvent and, after removal of undissolved plant parts, the extract formed thereby is subjected to an UV-absorption measurement wherein for a first wavelength range of 200-270 nm, an absorption signal is recorded. A second derivative of the absorption signal is then obtained for a second wavelength range of 240-250 nm and the value of the minimum of the second derivative is determined and, based on this value, a concentration value for the glucoraphanin in the plant is established.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.