Patent · US Active

Molecular imaging biomarkers

US10445880B2 · kind B2 · utility

2Cited by
0References
16Claims
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Key dates

Filing dateMar 22, 2018
Grant dateOct 15, 2019
Priority date
Expiry dateApr 27, 2038

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG06T2207/30096
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

Methods for label-free characterization of untagged molecules within a biological sample in-situ. The untagged molecules may be constituent of extracellular vesicles, and are excited in the biological sample with at least one wavelength band of light derived from a single stream of optical pulses. Light emitted by the untagged molecules by SHG, THG, 2PAF and 3PAF processes is detected. Separate measures of the biological sample corresponding to light emitted by the untagged molecules in each of the SHG, THG, 2PAF and 3PAF processes are derived. On that basis, normal extracellular vesicles may be differentiated from extracellular vesicles associated with a tumor on the basis of a specified signature of characteristics of images of SHG, THG, 2PAF and 3PAF processes.

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