Method for isolating microbial DNA
US10450558B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | May 7, 2015 |
| Grant date | Oct 22, 2019 |
| Priority date | — |
| Expiry date | Dec 3, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1017
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a new method for enriching and/or isolating nucleic acids from microbial cells which comprises filtering a liquid sample through a nucleic acid-binding matrix which has a pore size small enough to retain microbial cells, lysing the microbial cells on the matrix to release the nucleic acids from the microbial cells, binding the nucleic acids to the matrix and subsequently eluting the DNA. The invention also relates to a method for enriching and/or isolating nucleic acids from microbial cells which are present in a liquid sample that comprises microbial cells and higher eukaryotic cells and/or tissues. The invention also provides a cartridge for carrying out the methods of the invention. Finally, the invention relates to kits for carrying out the methods of the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.