Compositions and methods for improved encapsulation of functional proteins in polymeric vesicles
US10456452B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 30, 2016 |
| Grant date | Oct 29, 2019 |
| Priority date | — |
| Expiry date | Sep 16, 2036 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K9/1273
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Methods of preparing polymersome-encapsulated functional protein suspensions may include thermally blending an amount of a block copolymer with an amount of a low molecular weight polyethylene glycol (PEG) for at least 30 minutes, mixing and cooling a resulting PEG/polymer formulation to room temperature, adding an aliquot of a solution of the functional protein to a sample containing the PEG/polymer formulation, and performing at least three dilution steps in which polymersomes that are generated are progressively saturated with the functional protein. The aliquot of the solution of the functional protein added may have a to the PEG/polymer sample of around 0.5:1 to 1.5:1 by volume, and the thermal blending may be performed at 90-100° C.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.