Method of constructing sequencing library
US10456769B2 · kind B2 · utility
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18Claims
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Key dates
| Filing date | Aug 3, 2017 |
| Grant date | Oct 29, 2019 |
| Priority date | — |
| Expiry date | Aug 3, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B50/08
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Provided is a method of constructing a sequencing library. The method includes 1) providing a single-stranded DNA fragment from a biological sample; 2) subjecting the single-stranded DNA fragment to whole genomic amplification to obtain a whole genome amplification product; 3) fragmenting the whole genome amplification product using a transposase embedded with two adaptors to obtain a fragmented product with two adaptors respectively at two ends; and 4) amplifying the fragmented product with two adaptors respectively at two ends using a tag sequence and a pair of primers to obtain said sequencing library.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.