Method for obtaining high-yield, stable expression cell clones and antibody molecules obtained thereby
US10457747B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Mar 11, 2016 |
| Grant date | Oct 29, 2019 |
| Priority date | — |
| Expiry date | Jul 12, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2510/02
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Provided is a method for obtaining high-yield, stable expression cell clones from myeloma cell lines in a protein-free culture medium. The method is used for industrial production of a recombinant antibody, and includes three stage: (1) adapting to a protein-free culture medium, statically culturing cells at a low density, and gradually reducing a fat-rich supplement to a chemical culture medium; (2) adapting to a protein-free culture medium; culturing cells at a high density, and using a perfusion fermentation system in a laboratory scale; and (3) screening high-yield, stable expression cell clones from the cells after fermentation ends. The cell clone may be used to produce a humanized anti-NeuGcGM3 14F7 recombinant antibody.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.