Patent · US Active

Methods of purifying bispecific antibodies

US10457749B2 · kind B2 · utility

4Cited by

1References

16Claims

0Family size

Assignee

Novimmune SA · CH

Inventors

Nicolas Fouque · Saint-Julien-en-Genevois, FR
Jean François Depoisier · Bonneville, FR
Keith Wilson · Wabash, US
Judith Vajda · Leonberg, DE
Egbert Muller · Langen (Hessen), DE
Romain Dabre · Darmstadt, DE

Key dates

Filing date	Mar 14, 2016
Grant date	Oct 29, 2019
Priority date	—
Expiry date	May 6, 2037

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K2317/52
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention relates to the purification of bispecific antibodies carrying a different specificity for each binding site of the immunoglobulin molecule from a mixture of monospecific antibodies. The bispecific antibodies are composed of a single heavy chain and two different light chains, one containing a Kappa constant domain and the other a Lambda constant domain. This invention in particular relates to the isolation of these bispecific antibodies from mixtures that contain monospecific antibodies having two Kappa light chains or portions thereof and monospecific antibodies having two Lambda light chains or portions thereof. The invention also provides the methods of efficiently purifying these bispecific antibodies.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.