Efficient selectivity of recombinant proteins
US10457959B2 · kind B2 · utility
1Cited by
10References
33Claims
0Family size
Assignee
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Key dates
| Filing date | Jul 31, 2017 |
| Grant date | Oct 29, 2019 |
| Priority date | — |
| Expiry date | Jul 31, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides a new expression system comprising a mammalian selectable marker that promotes desirable post-translational modifications of glycoproteins. In particular, the invention includes methods and compositions for optimal recombinant protein expression in mammalian cells by employing a selection marker system based on GPT genes of mammalian origin. The invention includes methods that facilitate selectivity and enhanced expression copies as well as protein yield of recombinant proteins in mammalian cells, and methods of using GPT expression systems.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.