Patent · US Active

Polynucleotide enrichment using CRISPR-Cas systems

US10457969B2 · kind B2 · utility

1Cited by

0References

19Claims

0Family size

Assignee

Illumina, Inc. · US

Inventors

Gordon M. Cann · Redwood City, US
Jeffrey G. Mandell · Village of La Jolla, US
Alex Aravanis · San Francisco, US
Steven Norberg · San Diego, US
Dmitry K. Pokholok · San Diego, US
Frank J. Steemers · Encinitas, US
Farnaz Absalan · San Francisco, US
Leila Bazargan · San Diego, US

Key dates

Filing date	Jul 20, 2015
Grant date	Oct 29, 2019
Priority date	—
Expiry date	Jul 3, 2037

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q2521/301
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A method for enriching a target nucleic acid comprising providing an endonuclease system having a crRNA or a derivative thereof, and a Cas protein or a variant thereof. The crRNA or the derivative thereof contains a target-specific nucleotide region substantially complementary to a region of the target nucleic acid; contacting the target nucleic acid with the endonuclease system to form a complex; and separating the complex and thereby enriching for the target nucleic acid.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.