Lyase and method for asymmetric synthesis of (S)-phenylacetylcarbinol

US10465211B2 · kind B2 · utility

Assignee

• Forschungszentrum Jülich GmbH · DE

Inventors

• Doerte Rother · Gerstungen, DE
• Martina Pohl · Aachen, DE
• Torsten Sehl · Offenburg, DE
• Lisa Marx · Seeheim-Jugenheim, DE
• Robert Westphal · Opfingen, DE

Key dates

• Filing date: Aug 27, 2015
• Grant date: Nov 5, 2019
• Expiry date: Aug 27, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y401/01001
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A lyase has an amino acid sequence selected from SEQ ID NOs: 1, 2 and 3, wherein the amino acid isoleucine in position no. 468 in the protein ApPDC-E469G, which is modified with respect to the wild type from Acetobacter pasteurianus, is replaced by an amino acid which occupies less space than isoleucine.