Methods for quantifying efficiency of nucleic acid extraction and detection
US10465232B1 · kind B1 · utility
5Cited by
5References
11Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 7, 2016 |
| Grant date | Nov 5, 2019 |
| Priority date | — |
| Expiry date | May 24, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/689
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for quantifying the efficiency of nucleic acid extraction from a sample comprising a mixture of species are provided. In some embodiments, the method comprises adding an initial amount of one or more spike-ins to the sample, extracting nucleic acids from the sample, quantifying the amount of the one or more spike-ins in the extracted nucleic acid sample, and comparing the initial amount of the one or more spike-ins to the quantified amount of the one or more spike-ins.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.