Genetically encoded probe for quantification of lactate and methods for quantifying metabolic rates and lactate transport
US10466233B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Apr 13, 2012 |
| Grant date | Nov 5, 2019 |
| Priority date | — |
| Expiry date | Sep 14, 2033 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/582
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A nanosensor for detecting and quantifying lactate in different types of samples, such as tissues, intra-cellular and subcellular compartments, with high spatial and temporal resolution is disclosed. Methods comprising use of the nanosensor for quantifying the activity of lactate transporters, rates of cellular lactate production and cellular lactate consumption, and rate of mitochondrial pyruvate consumption are also disclosed. Methods for quantifying the transformation in energy metabolism that characterizes cancer cells with single-cell resolution and for detecting interference of candidate drugs with mitochondrial energetics are additionally disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.