High-density microchamber array and manufacturing method thereof
US10471429B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 18, 2014 |
| Grant date | Nov 12, 2019 |
| Priority date | — |
| Expiry date | Feb 7, 2036 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2300/165
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A material film is formed as a thin film having a thickness of 1 μm on a surface of a glass substrate. A plurality of micro-chambers having a diameter of 5 μm are formed in the material film to be arrayed at a high density. The respective chambers filled with an aqueous test solution have openings that are liquid-sealed by a lipid bilayer membrane to provide a high-density micro-chamber array. Significant downsizing of the micro-chambers enhances a change in concentration by a reaction of one biomolecule in the chamber and thereby increases the detection sensitivity. In the configuration that a large number of micro-chambers are formed at a high density, even in the case of an extremely slow reaction of the biomolecule, the reaction proceeds in any of the chambers. This configuration accordingly enables the reaction of the biomolecule to be detected with high sensitivity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.