Cardiac troponin I ultra-sensitive detection reagent kit, and ultra-sensitive detection method therefor

US10472400B2 · kind B2 · utility

Assignee

• Shenzhen New Industries Biomedical Engineering Co., Ltd. · CN

Inventors

• Wei Rao · Beijing, CN
• Jinyun YUAN · Nanhu, CN
• Qin Li · Houston, US
• Sheng Wang · Lanzhou, CN
• Hong Xu · San Diego, US
• Wu-Hsi Li · Somerville, US
• Tinghua LI · Kunming, CN
• Jinqiu Fu · Nanhu, CN

Key dates

• Filing date: Feb 10, 2015
• Grant date: Nov 12, 2019
• Expiry date: May 8, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07D401/14
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A cardiac troponin I ultra-sensitive detection reagent kit, a preparation method, and a detection method. The reagent kit comprises at least one first anti-cardiac troponin I antibody marked with a trace marker and at least one second anti-cardiac troponin I antibody coated on magnetic microspheres, the first anti-cardiac troponin I antibody and cardiac troponin I binding site being different from the second anti-cardiac troponin I antibody and cardiac troponin I binding site. The reagent kit may further comprise a diluent capable of significantly reducing non-specific binding in a detection process, so as to further increase the detection accuracy and sensitivity. The method using the reagent kit to detect cardiac troponin I sensitively and accurately detects the amount of cardiac troponin I in a sample, and provides more timely and reliable information for the early diagnosis and treatment of AMI.