Generation of a mesenchymal stromal cell bank from the pooled mononuclear cells of multiple bone marrow donors

US10472608B2 · kind B2 · utility

Assignees

• JOHANN WOLFGANG GOETHE-UNIVERSITAT · DE
• DRK BLUTSPENDEDIENST BADEN-WÜRTTEMBERG-HESSEN GGMBH · DE

Inventors

• Peter Bader · Hamburg, DE
• Selim Kuci · Lörrach, DE
• Zyrafete Kuci · Frankfurt am Main, DE
• Halvard Bönig · Frankfurt, DE

Key dates

• Filing date: Jul 14, 2015
• Grant date: Nov 12, 2019
• Expiry date: Jul 14, 2035

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K2035/124
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention pertains to an improved mesenchymal stromal cell (MSC) preparation and a method for producing the same. The invention provides a new strategy to isolate MSC from bone marrow mononuclear cells (BM-MNCs) by pooling BM-MNCs of multiple unrelated (third-party) bone marrow donors. The MSC preparation manufactured in accordance with the methodology of the invention is characterized by a stable proliferative capability and an increased immunosuppressive potential when compared to individual donor MSC preparations or a pool of individual MSCs generated from multiple donors. The MSCs prepared ac- cording to the invention are particularly useful for medical applications such as the treatment of graft-versus-host disease (GvHD) in recipients with hematopoietic stem cell transplants, patients with autoimmune disorders and as a cell-based therapy in regenerative medicine.