Method, substrate and device for separating nucleic acids

US10472620B2 · kind B2 · utility

Assignee

• General Electric Company · US

Inventors

• John Richard Nelson · Fryeburg, US
• David Roger Moore · Rexford, US
• Robert Scott Duthie · Schenectady, US
• Matthew Jeremiah Misner · Delanson, US
• Gregory Andrew Grossmann · Halfmoon, US
• Elizabeth Marie Dees · Ballston Lake, US
• Patrick McCoy Spooner · Slingerlands, US
• Erik Leeming Kvam · Albany, US
• Andrew Arthur Paul Burns · Niskayuna, US
• Vicki Herzl Watkins · Alplaus, US

Key dates

• Filing date: Jul 1, 2014
• Grant date: Nov 12, 2019
• Expiry date: Oct 1, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6834
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; and flowing a nucleic acid amplification reaction mixture across a length of the substrate through the sample application zone to amplify the target nucleic acid forming a nucleic acid amplification product; wherein the target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight migrates away from the sample application zone. An associated device is also provided.