Patent · US Active

System and method for targeted depletion of nucleic acids

US10472666B2 · kind B2 · utility

0Cited by

4References

11Claims

0Family size

Assignee

Roche Sequencing Solutions, Inc. · US

Inventors

Venera Bouriakov · Madison, US
Daniel Burgess · Black Earth, US

Key dates

Filing date	Feb 3, 2017
Grant date	Nov 12, 2019
Priority date	—
Expiry date	Feb 3, 2037

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y301/26004
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present disclosure provides a system and method for depleting target nucleic acids from a nucleic acid sample. In one aspect, a kit according to the present disclosure includes a plurality of DNA probes. Each of the DNA probes is hybridizable to form a heteroduplex with at least one of a plurality of target RNA transcripts in a nucleic acid sample. The number of unique target RNA transcripts hybridized by the plurality of DNA probes is at least three. The kit further includes an enzyme having RNA-DNA hybrid ribonucleotidohydrolase activity, where degrades at least the RNA portion of the heteroduplex.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.