Method for the generation of a monoclonal plant cell line

US10480001B2 · kind B2 · utility

Assignee

• Fraunhofer-Gesellschaft zur förderung der angewandten Forschung e.V. · DE

Inventors

• Janina Kirchhoff · Aachen, DE
• Stefan Schillberg · Aachen, DE
• Andreas Schiermeyer · Neunburg vorm Wald, DE
• Helga Schinkel · Aachen, DE
• Rainer Fischer · Monschau, DE

Key dates

• Filing date: Jun 7, 2011
• Grant date: Nov 19, 2019
• Expiry date: Oct 10, 2031

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A01H4/005
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides a method for the generation of a monoclonal plant cell line from a heterologous population of plant cells, comprising the following steps: (a) provision of a heterologous population of plant cells; (b) preparation of protoplasts from said heterologous population of plant cells; (c) separation of single protoplasts by subjecting the preparation of protoplasts to flow cytometric sorting; (d) regeneration of a separated single transformed protoplast until the formation of a microcolony by co-cultivation in the presence of feeder cell material; (e) removal of the microcolony from the feeder cell material and cultivation of the microcolony until the formation of a monoclonal plant cell line.