Method and automatic machine for embedding a tissue sample into an embedding medium

US10481054B2 · kind B2 · utility

Assignee

• Leica Biosystems Nussloch GmbH · DE

Inventors

• Florian Böhl · Neckargemünd, DE
• Ralf Eckert · Schriesheim, DE
• Karin Flieger · Seeheim-Jugenheim, DE
• Markus Berberich · Heidelberg, DE
• Hermann Ulbrich · Annaberg-Buchholz, DE
• Stella Knorr · Brighton, AU
• Andrew Guy · Melbourne, AU

Key dates

• Filing date: Sep 1, 2016
• Grant date: Nov 19, 2019
• Expiry date: May 22, 2037

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2001/366
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention relates to a method for embedding a tissue sample into an embedding medium. In a first step the tissue sample is held in an intended orientation in a container by means of a holding element that presses the tissue sample against a base of the container. In a further step a liquid embedding medium that has a temperature above 64 degrees Celsius, in particular in the range from 65 degrees Celsius to 67 degrees Celsius, or a temperature of 66 degrees Celsius, is poured into the container, and the base of the container is cooled. In a further step a separating motion is executed by way of which the tissue sample and the holding element move away from each other, those layers of the embedding medium through which the holding element moves during the separating motion having, during the separating motion, temperatures in the range from 54 degrees Celsius to 64 degrees Celsius, in particular of 60 degrees Celsius, and/or those portions of the embedding medium which are directly adjacent to the holding element having, during the separating motion, a temperature in the range from 54 degrees Celsius to 64 degrees Celsius, in particular of 60 degrees Celsius.