Protein sequencing method and reagents

US10481162B2 · kind B2 · utility

Assignee

• The Governing Council of the University of Toronto · CA

Inventors

• Andrew Emili · Needham, US
• Megan McLaughlin · Toronto, CA
• Kyrylo Zagorovsky · Toronto, CA
• Jonathan Buchanan Olsen · Kitchener, CA
• Warren C. W. Chan · Toronto, CA
• Sachdev Sidhu · Toronto, CA

Key dates

• Filing date: Sep 5, 2018
• Grant date: Nov 19, 2019
• Expiry date: Sep 5, 2038

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/952
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention describes methods and reagents useful for sequencing polypeptide molecules. The method comprises affixing a polypeptide to a substrate and contacting the polypeptide with a plurality of probes. Each probe selectively binds to an N-terminal amino acid or an N-terminal amino acid derivative. Probes bound to the polypeptide molecule are then identified before cleaving the N-terminal amino acid or N-terminal amino acid derivative of the polypeptide. Also provided are methods for the sequencing a plurality of polypeptide molecules in a sample and probes specific for N-terminal amino acids or N-terminal amino acid derivatives.