Stabilizing 5′-flap endonuclease activity
US10487317B1 · kind B1 · utility
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Key dates
| Filing date | Mar 23, 2017 |
| Grant date | Nov 26, 2019 |
| Priority date | — |
| Expiry date | Feb 15, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y301/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A probe qPCR master mix is provided. In some embodiments, the master mix comprises nucleotides, an enzyme comprising a polymerase activity and a flap endonuclease activity, a chelating agent at a concentration greater than 5 μM, and a divalent cation. The relatively high concentration of chelating agent stabilizes the flap endonuclease activity during storage. As such, the polymerase and flap endonuclease activities may be substantially the same before and after storing the master mix for 7 days at 37° C.
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