One-pot multiplex gene synthesis
US10487323B2 · kind B2 · utility
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40Claims
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Key dates
| Filing date | Aug 3, 2015 |
| Grant date | Nov 26, 2019 |
| Priority date | — |
| Expiry date | Aug 3, 2035 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01J2219/00722
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides methods for generating a library of synthetic polynucleotides. The present invention also provides methods for generating proteins encoded by the library of synthetic polynucleotides. In addition, provided herein are methods for determining the soluble expression of said proteins. This invention is based, in part, on the discovery of a method for selecting optimal oligonucleotides in combination with performing a phosphorylation reaction, ligation reaction and PCR amplification in a single reaction vessel to produce synthetic polynucleotides in a multiplex manner.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.