Methods of nucleic acid analysis using terminator nucleotides

US10487357B2 · kind B2 · utility

Assignee

• Life Technologies Corporation · US

Inventors

• Wolfgang Hinz · Killingworth, US
• Steven M. Menchen · Fremont, US
• Ronald Graham · Pleasanton, US
• Peter Vander Horn · Foster City, US
• Earl Hubbell · Mountain View, US
• Christian Woehler · Darmstadt, DE
• Roman Rozhkov · Carlsbad, US
• Barnett Rosenblum · San Jose, US

Key dates

• Filing date: Mar 18, 2016
• Grant date: Nov 26, 2019
• Expiry date: Mar 18, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6874
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

In some embodiments, the disclosure relates generally to methods, as well as related, systems, compositions, kits and apparatuses, for nucleic acid analysis that involve the use of modified nucleotides, including terminator nucleotides and/or tagged nucleotides, in a template-dependent nucleotide incorporation reaction. In some embodiments, the nucleic acid analysis can be conducted at a single reaction site, or at a plurality of reaction sites in an array of reaction sites. Optionally, the array contains a plurality of reaction sites having about 1-100 million, or about 100-250 million, or about 200-500 million, or about 500-900 million, or more reaction sites. Optionally, each reaction site is in contact with, operatively coupled, or capacitively coupled to one or more sensors that are ion-sensitive FETs (isFETs) or chemically-sensitive FETs (chemFETs) sensors. Optionally, the reaction sites are in fluid communication with each other.