Buffer-and-salt-content-independent UV-VIS spectroscopic characterisation of sample DNA and/or RNA content using reference samples having distinct buffer and/or salt content
US10488330B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Aug 30, 2013 |
| Grant date | Nov 26, 2019 |
| Priority date | — |
| Expiry date | Mar 9, 2036 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method is described for characterising a sample comprising at least DNA and/or RNA. The method comprises obtaining a UV-VIS spectroscopic measurement of the sample comprising at least DNA and/or RNA, deriving an absorption parameter based on the UV-VIS spectroscopic measurement, and determining a DNA and/or RNA quantification present in the sample. Determining a DNA and/or RNA quantification thereby comprises taking into account a buffer and/or salt effect on the UV-VIS spectroscopic measurement irrespective of the actual buffer and salt content of the sample, based on said derived absorption parameter and reference spectral data including spectral information for buffer and/or salt effects.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.