Simple method and kit for DNA typing of HLA genes by high-throughput massively parallel sequencer
US10494673B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 27, 2014 |
| Grant date | Dec 3, 2019 |
| Priority date | — |
| Expiry date | Nov 27, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6881
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention addresses the problem of providing a method and kit for the DNA profiling of HLA genes using a high-throughput massively parallel sequencer. The present invention pertains to a method for the DNA profiling of HLA genes, said method being characterized by including: (1) a step for preparing a primer set that anneals specifically to exon 4 and intron 1 and includes exon 2 and exon 3 of at least one target gene selected from the group consisting of HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5, HLA-DQB1 and HLA-DPB1 in the base sequence of the human genome; (2) a step for amplifying a sample (DNA) by PCR using the primer set; (3) a step for determining the base sequence of the amplified PCR product; and (4) a step for carrying out a homology search against a database.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.