Method for quantitative monitoring of endospores in aqueous environment of a paper or board mill
US10494679B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 26, 2016 |
| Grant date | Dec 3, 2019 |
| Priority date | — |
| Expiry date | Sep 3, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/24
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a method for quantitative monitoring of bacterial endospores in an aqueous environment of a paper or board mill. The method comprises at least the following steps: obtaining at least a first aqueous sample originating from the industrial aqueous environment; destroying bacteria in vegetative form in the first sample by a suitable treatment, preferably by heating the first sample to a desired temperature; adding intercalating agent (such as PMA) to the treated first sample and allowing it to interact (e.g. by cross-linking) with the destroyed bacteria, so that the nucleic acid from the destroyed bacteria are unavailable for PCR; and determining the endospore level in the first sample by using quantitative polymerase chain reaction (qPCR) in which only the DNA from the endospores is available for amplification.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.