Method and system for imaging and analysis of a biological specimen

US10495554B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Karl Deisseroth · Stanford, US
• William E. Allen · Peoria, US
• Brian Hsueh · Pacifica, US
• Li Ye · Ningbo City, CN

Key dates

• Filing date: May 25, 2017
• Grant date: Dec 3, 2019
• Expiry date: May 25, 2037

Classification

• Technology area (CPC G): Physics
• CPC primary: G06T2207/30024
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present disclosure provides methods of preparing a biological specimen for imaging analysis, comprising fixing and clearing the biological specimen and subsequently analyzing the cleared biological specimen using microscopy. Also included are methods of quantifying cells, for example, active populations of cells in response to a stimulant. The present disclosure also provides devices for practicing the described methods. A flow-assisted clearing device provides rapid clearing of hydrogel-embedded biological specimens without the need of specialized equipment such as electrophoresis or perfusion devices.